Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code See Below
Description Legionella is characterized as gram-negative, aerobic, encapsulated bacilli. Legionnaires' disease, or Legionellosis, is a severe infection caused by Legionella species, primarily L. pneumophila which is responsible for 90% of infections. The disease typically presents as pneumonia and symptoms may include a high fever, chills, cough, muscle aches, headaches, and diarrhoea. Legionella species is also linked to Pontiac fever, which is an acute, febrile, self-limited illness. The infection is generally characterized by fever, fatigue, and muscle pain.

The bacterium is found in environmental water sources, potable water, such as that from faucets, showers, and hot water tanks and cooling towers, and hot tubs. Legionella infection can occur in a variety of hosts. Modes of transmission of Legionella include the aspiration of water contaminated with the organism and inhalation of aerosols containing Legionella. Although uncommon in animals, predisposing factors such as poor hygiene, bad management, and insufficient and/or unbalanced feeding seem to be important in the occurrence of the disease.

Pathogens Tested
  • APL-035 : Legionella Species (This Assay includes the detection of Legionella Species only. This Assay can detect Legionella pneumophila, L. adelaidensis, L. anisa, L. birminghamensis, L. bozemanii , L. brunensis, L. cherii , L. cincinnatiensis, L. dumoffii, L. erythra , L. feelei, L. gormanii, L. gratiana, L. hackeliae, L.israelensis, L. jamestownensis, L. jordanis, L. lansingensis, L. longbeachae, L. maceachernii , L. micdadei , L. moravica, L. oakridgensis paucimobilis , L. parisiensis, L. quinlivanii, L. rubrilucens, L. sainthelensis, L. spiritensis, L. steigerwaltii , L. taurinensis, L. tucsonensis, L. wadsworthii)
  • APL-034 : Legionella pneumophila (This Assay includes the detection of Legionella species and subtype Legionella pneumophila.)

Method Real-Time PCR.
Sample Type
Urine, Swab/Secretion (Respiratory), Water, Culture.
Transport Condition Samples should be transported at 4°C. Urine sample must be frozen after collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APL-036
Description Leptospirosis is a transmissible disease of animals caused by infection with any of the pathogenic members of the genus Leptospira, which are aerobic, right-handed helical bacteria. Leptospirosis has a global distribution, and leptospires have been detected in more than 180 species of animals. Mammals are the only class of animals capable of transmitting Leptospira organisms, even though leptospires have been identified in reptiles and birds.

Clinical symptoms of acute leptospirosis include: sudden onset of agalactia (in adult milking cattle and sheep); icterus and haemoglobinuria, especially in young animals; meningitis; and acute renal failure or jaundice in dogs. Chronic leptospirosis results in abortion, stillbirth, birth of weak offspring (premature), infertility, chronic renal failure or chronic active hepatitis in dogs; and cases of periodic ophthalmia in horses.

Method Real-Time PCR
Sample Type
Accredited : EDTA Blood, Tissue (liver, lung, brain, kidney), Culture.
Alternatives : Urine, Milk, Cerebrospinal Fluid, Thoracic Fluid, Peritoneal Fluid.
Transport Condition Samples should be transported at 4°C. Urine sample must be frozen after collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code See Below
Description Legionella is characterized as gram-negative, aerobic, encapsulated bacilli. Legionnaires' disease, or Legionellosis, is a severe infection caused by Legionella species, primarily L. pneumophila which is responsible for 90% of infections. The disease typically presents as pneumonia and symptoms may include a high fever, chills, cough, muscle aches, headaches, and diarrhoea. Legionella species is also linked to Pontiac fever, which is an acute, febrile, self-limited illness. The infection is generally characterized by fever, fatigue, and muscle pain.

The bacterium is found in environmental water sources, potable water, such as that from faucets, showers, and hot water tanks and cooling towers, and hot tubs. Legionella infection can occur in a variety of hosts. Modes of transmission of Legionella include the aspiration of water contaminated with the organism and inhalation of aerosols containing Legionella. Although uncommon in animals, predisposing factors such as poor hygiene, bad management, and insufficient and/or unbalanced feeding seem to be important in the occurrence of the disease.

Pathogens Tested
  • APL-035 : Legionella Species (This Assay includes the detection of Legionella Species only. This Assay can detect Legionella pneumophila, L. adelaidensis, L. anisa, L. birminghamensis, L. bozemanii , L. brunensis, L. cherii , L. cincinnatiensis, L. dumoffii, L. erythra , L. feelei, L. gormanii, L. gratiana, L. hackeliae, L.israelensis, L. jamestownensis, L. jordanis, L. lansingensis, L. longbeachae, L. maceachernii , L. micdadei , L. moravica, L. oakridgensis paucimobilis , L. parisiensis, L. quinlivanii, L. rubrilucens, L. sainthelensis, L. spiritensis, L. steigerwaltii , L. taurinensis, L. tucsonensis, L. wadsworthii)
  • APL-034 : Legionella pneumophila (This Assay includes the detection of Legionella species and subtype Legionella pneumophila.)

Method Real-Time PCR.
Sample Type
Urine, Swab/Secretion (Respiratory), Water, Culture.
Transport Condition Samples should be transported at 4°C. Urine sample must be frozen after collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Accredited
Assay Code APL-036
Description Leptospirosis is a transmissible disease of animals caused by infection with any of the pathogenic members of the genus Leptospira, which are aerobic, right-handed helical bacteria. Leptospirosis has a global distribution, and leptospires have been detected in more than 180 species of animals. Mammals are the only class of animals capable of transmitting Leptospira organisms, even though leptospires have been identified in reptiles and birds.

Clinical symptoms of acute leptospirosis include: sudden onset of agalactia (in adult milking cattle and sheep); icterus and haemoglobinuria, especially in young animals; meningitis; and acute renal failure or jaundice in dogs. Chronic leptospirosis results in abortion, stillbirth, birth of weak offspring (premature), infertility, chronic renal failure or chronic active hepatitis in dogs; and cases of periodic ophthalmia in horses.

Method Real-Time PCR
Sample Type
Accredited : EDTA Blood, Tissue (liver, lung, brain, kidney), Culture.
Alternatives : Urine, Milk, Cerebrospinal Fluid, Thoracic Fluid, Peritoneal Fluid.
Transport Condition Samples should be transported at 4°C. Urine sample must be frozen after collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links